Coding

Part:BBa_K3113051:Design

Designed by: Sarah Brajkovic, Joshua Hesse   Group: iGEM19_Munich   (2019-09-05)


CD63_Ser161_6xHis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The His-Tag was cloned on the exosomal marker on the large extracellular loop for purification of exosomes. Specifically, the 6 histidines were added after position Ser161 based on a structural model for CD63[1] generated with swissmodel.expasy.org[2] and based on the structure of the related tetraspanin CD81[3].

Source

This sequence has its origin in Homo sapiens. The gene is located on the human chromosome 12q13.

References

  1. ↑ https://2019.igem.org/wiki/images/0/02/T--Munich--CD63_Structure_pdf.pdf
  2. ↑ https://swissmodel.expasy.org
  3. ↑ https://swissmodel.expasy.org/templates/5tcx.1